Data/hora: 03/06/2024 15:10:12

Provedor de dados: 	Brazilian Journal of Genetics	
País: 	Brazil	
Título: 	A 37-kb restriction map of the human immunoglobulin lambda variable locus, VB cluster, harboring four functional genes and two non-coding Vl sequences	
Autores: 	Passos Jr.,Geraldo A.S.; Lefranc,Marie-Paule.	
Data: 	1997-12-01	
Ano: 	1997	
Resumo: 	The human immunoglobulin lambda variable locus (IGLV) is mapped at chromosome 22 band q11.1-q11.2. The 30 functional germline v-lambda genes sequenced untill now have been subgrouped into 10 families (V&lt;FONT FACE="Symbol"&gt;l&lt;/font&gt;1 to V&lt;FONT FACE="Symbol"&gt;l&lt;/font&gt;10). The number of V&lt;FONT FACE="Symbol"&gt;l&lt;/font&gt; genes has been estimated at approximately 70. This locus is formed by three gene clusters (VA, VB and VC) that encompass the variable coding genes (V) responsible for the synthesis of lambda-type Ig light chains, and the J&lt;FONT FACE="Symbol"&gt;l&lt;/font&gt;-C&lt;FONT FACE="Symbol"&gt;l&lt;/font&gt; cluster with the joining segments and the constant genes. Recently the entire variable lambda gene locus was mapped by contig methodology and its one- megabase DNA totally sequenced. All the known functional V-lambda genes and pseudogenes were located.  We screened a human genomic DNA cosmid library and isolated a clone with an insert of 37 kb (cosmid 8.3) encompassing four functional genes (IGLV7S1, IGLV1S1, IGLV1S2 and IGLV5a), a pseudogene (V&lt;FONT FACE="Symbol"&gt;l&lt;/font&gt;A) and a vestigial sequence (vg1) to study in detail the positions of the restriction sites surrounding the V&lt;FONT FACE="Symbol"&gt;l&lt;/font&gt; genes. We generated a high resolution restriction map, locating 31 restriction sites in 37 kb of the VB cluster, a region rich in functional V&lt;FONT FACE="Symbol"&gt;l&lt;/font&gt; genes. This mapping information opens the perspective for further RFLP studies and sequencing	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000400027	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S0100-84551997000400027	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Genetics  v.20 n.4 1997	
Fonte: 	info:eu-repo/semantics/openAccess